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KMID : 0613820120220081107
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Journal of Life Science
2012 Volume.22 No. 8 p.1107 ~ p.1113
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Effect of Pueraria thunbergiana Extracts on the Activation of Immune Cells
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Kim Jong-Jin
Lee Hyeok-Jae
Yee Sung-Tae
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Abstract
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In this experiment, the effects of Pueraria thunbergiana extracts on the activation of immune cells were studied. An immune cell-activating factor was partially purified from P. thunbergiana by means of physiological saline extraction, acetone precipitation, and heating inactivation. P. thunbergiana extracts increased the proliferation of spleen cells and induced the production of IL-2, IL-6, TNF-α, and IFN-r by spleen cells. Also, they increased the proliferation of purified B cells and the production of IgM antibody in a dose-dependent fashion. The extract self-induced NO synthesis in a mouse macrophage cell line (RAW264.7). When cell lines were treated with extracts, the cytokines' (IL-1β, IL-6, and TNF-α) production was markedly increased. Therefore, P. thunbergiana extract can self-activate spleen cells, B cells, and macrophages. These results might be useful in further studies into a possible immune-activating agent derived from P. thunbergiana for the development of functional foods and drugs.
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KEYWORD
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Pueraria thunbergiana, cytokines, B cells, nitric oxide (NO), macrophage
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